On the basis of analysis at 22 loci, coding allozyme diversity of MDH, GOT, SKDH, LAP, 6-PGD, IDH, FDH, PGM, GDH, SDH, PEPCA и ADH, the data about genetic structure, levels of variability and degree of differentiation Scotch pine (Pinus sylvestris L.) growing in south-taiga subzone of West Siberian lowland on swamps with distinct types of water-mineral nutrition (eutrophic, mesotrophic, oligotrophic) and on upland were obtained. It was studied eight of pine cenopopulations including one population on eutrophic swamp, two populations on mesotrophic swamps, four populations on oligotrophic swamps and one population on upland. Seven of them were situated on territory of Timiryazev leskhoz and one population was situated on territory of Bakchar leskhoz in Tomsk region. In each population 29-30 trees were analyzed. It was established that all studied pine populations are characterized enough high level of genetic diversity. The percentage of polymorphic loci at the 100% criterion (P) ranges from 63.64% to 72.73%, the mean number of alleles per locus (A) ranges from 1.82 to 2.41, the observed (Ho) and the expected (He) heterozygosities range from 0.182 to 0.230 and from 0.191 to 0.212, respectively. The effective number of alleles (ne) is from 1.32 to 1.37. The greatest allelic diversity and level of heterozygosity are observed in population of pine located on mesotrophic site of oligotrophic swamp (A=2.41, Ho=0.230, He=0.212, ne=1.37). As a whole in joint sampling of populations about 73% of assayed loci are polymorphic and each tree is heterozygous for 20% of its genes. The mean number of alleles per locus is equal to 2.73, the effective number of alleles is equal to 1.35. The obtained values are somewhat higher than those established for pine populations from various on geographic location regions of Krasnoyarsk krai, Khakasiya and Tuva (P=68.18%, Ho=0.190, He=0.191, A=2.45, ne=1.33) studied at the same set of loci. In three populations studied the deficiency of heterozygous genotypes in comparison with equilibrium of Hardy-Weinberg is observed whereas in remaining populations the excess of heterozygotes is observed. On average each tree has 1.5% excess of heterozygotes (Fis=-0.015) relative to the population and 1.4% deficiency of heterozygotes (Fit=0.014) relative to the species as a whole. The low mean values of coefficients of inbreeding Fis and Fit testify to Scotch pine in investigated part of area is in the state close to equilibrium. The analysis of interpopulation differentiation by means of coefficient of inbreeding Fst showed that 97.2% of genetic variation revealed in populations refer to intrapopulation variation and only 2.8% refer to interpopulation variation. The values of Fst for various loci ranges from 0.006 (6-Pgd-2) to 0.106 (Got-1). The genetic distance D (Nei, 1972) between populations varies from 0.003 to 0.015. In spite of the low on average differentiation of populations studied (D=0.007) the differences in genetic structure between some of compared pairs of swamp populations are observed. The most significant differences are revealed between population of pine on eutrophic swamp and populations on oligotrophic swamps. The genetic distance between them compound 0.012, on average. The populations of pine situated on various oligotrophic swamps are differentiated far less (D=0.009). The obtained results testify to heterogeneity of water-mineral nutrition conditions exert influence on degree of genetic differentiation swamp populations of Scotch pine.

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